• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TTC4 Polyclonal antibody

TTC4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11878-1-AP

Synonyms

TPR repeat protein 4, TTC4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using TTC4 Polyclonal antibody (11878-1-AP)

    WB analysis of HeLa using 11878-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11878-1-AP (TTC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TTC4 Polyclonal antibody (11878-1-AP)

    WB analysis of HEK-293 using 11878-1-AP

    WB result of TTC4 antibody (11878-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC4 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using TTC4 Polyclonal antibody (11878-1-AP)

    WB analysis of HEK-293 using 11878-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11878-1-AP (TTC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TTC4 Polyclonal antibody (11878-1-AP)

    WB analysis of HeLa using 11878-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11878-1-AP (TTC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using TTC4 Polyclonal antibody (11878-1-AP)

    IHC staining of human lymphoma using 11878-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11878-1-AP (TTC4 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11878-1-AP targets TTC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC4 fusion protein Ag2474
Full Name tetratricopeptide repeat domain 4
Calculated Molecular Weight 387 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC001276
Gene Symbol TTC4
Gene ID (NCBI) 7268
RRIDAB_2211700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TTC4 antibody 11878-1-APDownload protocol
IHC protocol for TTC4 antibody 11878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Coffee (Verified Customer) (03-20-2022)

Verified by knockdown at expected size, but there is an additional non-specific band underneath it.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: K562 cell
TTC4 Antibody Western Blot validation (1:1000 dilution) in K562 cell (Cat no:11878-1-AP)