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TTC5 Polyclonal antibody

TTC5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26112-1-AP

Synonyms

Strap, TPR repeat protein 5, TTC5

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TTC5 Polyclonal antibody (26112-1-AP)

    WB analysis of Jurkat using 26112-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TTC5 Polyclonal antibody (26112-1-AP)

    WB analysis of Jurkat using 26112-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using TTC5 Polyclonal antibody (26112-1-AP)

    WB analysis of RAW 264.7 using 26112-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TTC5 Polyclonal antibody (26112-1-AP)

    IHC staining of human colon cancer using 26112-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TTC5 Polyclonal antibody (26112-1-AP)

    IHC staining of human colon cancer using 26112-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TTC5 Polyclonal antibody (26112-1-AP)

    IHC staining of mouse brain using 26112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TTC5 Polyclonal antibody (26112-1-AP)

    IHC staining of mouse brain using 26112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TTC5 Polyclonal antibody (26112-1-AP)

    IHC staining of mouse brain using 26112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, RAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26112-1-AP targets TTC5 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC5 fusion protein Ag23523
Full Name tetratricopeptide repeat domain 5
Observed Molecular Weight 45 kDa~49 kDa
GenBank Accession NumberBC053538
Gene Symbol TTC5
Gene ID (NCBI) 91875
RRIDAB_2880384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TTC5 antibody 26112-1-APDownload protocol
IHC protocol for TTC5 antibody 26112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols