TTC7A Polyclonal antibody

TTC7A Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 21600-1-AP

Synonyms

KIAA1140, TPR repeat protein 7A, TTC7, TTC7A



Tested Applications

Positive WB detected inK-562 cells, PC-3 cells, mouse small intestine tissue, mouse colon tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21600-1-AP is TTC7A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC7A fusion protein Ag16212
Full Name tetratricopeptide repeat domain 7A
Calculated Molecular Weight 858 aa, 96 kDa
Observed Molecular Weight 96 kDa
GenBank Accession NumberBC114365
Gene Symbol TTC7A
Gene ID (NCBI) 57217
RRIDAB_10733221
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTC7A (TPR repeat protein 7A) is expressed in enterocytes within the duodenum, ileum, and colon, and has a role in enterocyte survival and function. Mutations in the TTC7A gene can result in a spectrum of intestinal disease, including multiple intestinal atresia (MIA) and very early onset inflammatory bowel diseases (VEOIBD). Functional analysis revealed that TTC7A binds to and facilitates the transport of PI4KIIIa from the trans-Golgi to the plasma membrane, while loss of TTC7A results in dysregulation of PI4KIIIa signaling. This antibody specially recognizes endogenous TTC7A. (24417819)

Protocols

Product Specific Protocols
WB protocol for TTC7A antibody 21600-1-APDownload protocol
IHC protocol for TTC7A antibody 21600-1-APDownload protocol
IP protocol for TTC7A antibody 21600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Gastroenterology

Mutations in tetratricopeptide repeat domain 7A result in a severe form of very early onset inflammatory bowel disease.

Authors - Yaron Avitzur
humanWB,IHC

Front Immunol

A Novel Homozygous TTC7A Missense Mutation Results in Familial Multiple Intestinal Atresia and Combined Immunodeficiency.

Authors - Wenjun Mou
humanIHC

J Clin Immunol

Bialellic Mutations in Tetratricopeptide Repeat Domain 7A (TTC7A) Cause Common Variable Immunodeficiency-Like Phenotype with Enteropathy.

Authors - Dylan Lawless