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transcription termination factor-Specific Monoclonal antibody

transcription termination factor-Specific Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

7B3H2

Cat no : 60235-1-Ig

Synonyms

TTF 1, TTF I, TTF1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using transcription termination factor-Specific Monoclon (60235-1-Ig)

    WB analysis of HeLa using 60235-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60235-1-Ig (transcription termination factor-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHela cell

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60235-1-Ig targets transcription termination factor-Specific in WB,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name transcription termination factor, RNA polymerase I
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 106 kDa
GenBank Accession NumberNM_007344
Gene Symbol TTF1
Gene ID (NCBI) 7270
RRIDAB_2881359
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTF1 is a multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. It plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5, TTF1 recovers DNA-binding activity. The antibody is specific to TTF1.

Protocols

Product Specific Protocols
WB protocol for transcription termination factor-Specific antibody 60235-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols