Prealbumin/transthyretin Monoclonal antibody
Prealbumin/transthyretin Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1H8C4
Cat no : 66108-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Human brain, fetal tissue, human heart tissue |
| Positive IHC detected in | human liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
The immunogen of 66108-1-Ig is Prealbumin/transthyretin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Prealbumin/transthyretin fusion protein Ag19232 |
| Full Name | transthyretin |
| Calculated Molecular Weight | 147 aa, 16 kDa |
| Observed Molecular Weight | 14-16 kDa, 28-33 kDa |
| GenBank Accession Number | BC020791 |
| Gene Symbol | TTR |
| Gene ID (NCBI) | 7276 |
| RRID | AB_2881507 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. TTR can be expressed as monomer (14-16 kDa), dimmer (28-33 kDa), or tetramer around 55-60 kDa. (PMID: 22103483)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Prealbumin/transthyretin antibody 66108-1-Ig | Download protocol |
| IHC protocol for Prealbumin/transthyretin antibody 66108-1-Ig | Download protocol |
| FC protocol for Prealbumin/transthyretin antibody 66108-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Pathol Renal function in aged C57BL/6J mice is impaired by deposition of age-related apolipoprotein A-II amyloid independent of kidney aging |
