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TUBGCP2 Polyclonal antibody

TUBGCP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25856-1-AP

Synonyms

GCP 2, GCP2, h103p, hGCP2, hGrip103, hSpc97, SPBC97, Spc97p, TUBGCP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TUBGCP2 Polyclonal antibody (25856-1-AP)

    WB analysis using 25856-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25856-1-AP (TUBGCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using TUBGCP2 Polyclonal antibody (25856-1-AP)

    IP experiment of HeLa using 25856-1-AP

    IP result of anti-TUBGCP2 (IP:25856-1-AP, 4ug; Detection:25856-1-AP 1:500) with HeLa cells lysate 3920 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TUBGCP2 Polyclonal antibody (25856-1-AP)

    IHC staining of mouse testis using 25856-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25856-1-AP (TUBGCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TUBGCP2 Polyclonal antibody (25856-1-AP)

    IHC staining of mouse testis using 25856-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25856-1-AP (TUBGCP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, U2OS cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

25856-1-AP targets TUBGCP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TUBGCP2 fusion protein Ag23066
Full Name tubulin, gamma complex associated protein 2
Calculated Molecular Weight 902 aa, 103 kDa
Observed Molecular Weight 103 kDa
GenBank Accession NumberBC093770
Gene Symbol TUBGCP2
Gene ID (NCBI) 10844
RRIDAB_2880271
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUBGCP2 (tubulin-gamma-complex-associated protein 2) s TUBGCP2 is a core component of the g-TuRC nucleation complex (PMID: 3345861). Pathogenic variants in TUBGCP2 cause an autosomal recessive neurodevelopmental trait consisting of a neuronal migration disord (PMID: 31630790).

Protocols

Product Specific Protocols
WB protocol for TUBGCP2 antibody 25856-1-APDownload protocol
IHC protocol for TUBGCP2 antibody 25856-1-APDownload protocol
IP protocol for TUBGCP2 antibody 25856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Cell Proteomics

Interactome analysis reveals a link of the novel ALMS1-CEP70 complex to centrosomal clusters

Authors - Franziska Woerz
View Article