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  • KD/KO Validated

TXNDC5 Polyclonal antibody

TXNDC5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19834-1-AP

Synonyms

EndoPDI, ER protein 46, ERP46, Hcc 2, PDIA15, Thioredoxin like protein p46, TLP46, TXNDC5, UNQ364



Tested Applications

Positive WB detected inHepG2 cells, human skeletal muscle tissue, COLO 320 cells, mouse colon tissue, human placenta tissue
Positive IHC detected inhuman stomach cancer tissue, human stomach tissue, human colon cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19834-1-AP targets TXNDC5 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TXNDC5 fusion protein Ag13808
Full Name thioredoxin domain containing 5 (endoplasmic reticulum)
Calculated Molecular Weight 432 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC001199
Gene Symbol TXNDC5
Gene ID (NCBI) 81567
RRIDAB_10644285
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TXNDC5 antibody 19834-1-APDownload protocol
IHC protocol for TXNDC5 antibody 19834-1-APDownload protocol
IF protocol for TXNDC5 antibody 19834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF,IP

Sci Adv

Targeting mechanosensitive endothelial TXNDC5 to stabilize eNOS and reduce atherosclerosis in vivo.

Authors - Chih-Fan Yeh
  • KD Validated
human,mouseWB,IHC,IF

Nat Commun

Fibroblast-enriched endoplasmic reticulum protein TXNDC5 promotes pulmonary fibrosis by augmenting TGFβ signaling through TGFBR1 stabilization.

Authors - Tzu-Han Lee
  • KO Validated
mouseIHC,IF,WB,CoIP,FC

J Clin Invest

Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGFβ signaling in kidney fibroblasts.

Authors - Yen-Ting Chen
  • KD Validated
mouseWB,CoIP

Int J Mol Sci

Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation in the Hepatic AML12 Cell Line

Authors - Seyed Hesamoddin Bidooki
mouseWB

Antioxidants (Basel)

Counteracting Cisplatin-Induced Testicular Damages by Natural Polyphenol Constituent Honokiol.

Authors - Tse-En Wang
mouseWB

Antioxidants (Basel)

Squalene Loaded Nanoparticles Effectively Protect Hepatic AML12 Cell Lines against Oxidative and Endoplasmic Reticulum Stress in a TXNDC5-Dependent Way.

Authors - Seyed Hesamoddin Bidooki