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TXNDC9 Polyclonal antibody

TXNDC9 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25208-1-AP

Synonyms

APACD, PHLP3, Protein 1 4, TXNDC9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TXNDC9 Polyclonal antibody (25208-1-AP)

    WB analysis of HeLa using 25208-1-AP

    WB result of TXNDC9 antibody (25208-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC9 transfected HeLa cells.

  • Western Blot (WB) analysis of Raji cells using TXNDC9 Polyclonal antibody (25208-1-AP)

    WB analysis of Raji using 25208-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 25208-1-AP (TXNDC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TXNDC9 Polyclonal antibody (25208-1-AP)

    WB analysis of HEK-293 using 25208-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25208-1-AP (TXNDC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TXNDC9 Polyclonal antibody (25208-1-AP)

    WB analysis of K-562 using 25208-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25208-1-AP (TXNDC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TXNDC9 Polyclonal antibody (25208-1-AP)

    IF Staining of HEK-293 using 25208-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25208-1-AP (TXNDC9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inRaji cells, HEK-293 cells, K-562 cells, HeLa cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25208-1-AP targets TXNDC9 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TXNDC9 fusion protein Ag19509
Full Name thioredoxin domain containing 9
Calculated Molecular Weight 226 aa, 27 kDa
Observed Molecular Weight 25-27 kDa
GenBank Accession NumberBC005968
Gene Symbol TXNDC9
Gene ID (NCBI) 10190
RRIDAB_2879961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNDC9, also known as APACD, has a negative effect on protein folding by significantly reducing the activity of chaperone protein TCP1 complex ATPase activity. Including actin or tubulin.

Protocols

Product Specific Protocols
WB protocol for TXNDC9 antibody 25208-1-APDownload protocol
IF protocol for TXNDC9 antibody 25208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols