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Trappc9,NIBP Polyclonal antibody

Trappc9,NIBP Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 20176-1-AP

Synonyms

1810044A24Rik, 2900005P22Rik, 4632408O18Rik, Ibp, KIAA1882, mKIAA1882, NIBP, Trappc9, TRS130

Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using Trappc9,NIBP Polyclonal antibody (20176-1-AP)

    WB analysis of Neuro-2a using 20176-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Trappc9,NIBP Polyclonal antibody (20176-1-AP)

    WB analysis of mouse skeletal muscle using 20176-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Trappc9,NIBP Polyclonal antibody (20176-1-AP)

    WB analysis of mouse brain using 20176-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using Trappc9,NIBP Polyclonal antibody (20176-1-AP)

    WB analysis of SH-SY5Y using 20176-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNeuro-2a cells, mouse brain tissue, mouse skeletal muscle tissue, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20176-1-AP is Trappc9,NIBP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Trappc9,NIBP fusion protein Ag14018
Full Name trafficking protein particle complex 9
Calculated Molecular Weight 129/250/128 kDa
Observed Molecular Weight 128 kDa, 139 kDa
GenBank Accession NumberBC070463
Gene Symbol Trappc9
Gene ID (NCBI) 76510
RRIDAB_10694434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPPC9, also named as KIAA1882 and NIBP, belongs to the NIBP family. It functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. TRAPPC9 may function in neuronal cells differentiation. It may play a role in vesicular transport from endoplasmic reticulum to Golgi. TRAPPC9 was found in neurons of the cerebral cortex, hippocampus, and deep gray matter. In WB, the MW of TRAPPC9 is 130-140kd. A 250kd maybe the TRAPPC9 undergoes extensive posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for Trappc9,NIBP antibody 20176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols