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  • KD/KO Validated

UBA2 Polyclonal antibody

UBA2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15347-1-AP

Synonyms

ARX, HRIHFB2115, SAE2, UBA2, UBLE1B



Tested Applications

Positive WB detected inA549 cells, human brain tissue, Jurkat cells, HeLa cells, K-562 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15347-1-AP targets UBA2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBA2 fusion protein Ag7586
Full Name ubiquitin-like modifier activating enzyme 2
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC003153
Gene Symbol UBA2
Gene ID (NCBI) 10054
RRIDAB_2211586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBA2 antibody 15347-1-APDownload protocol
IHC protocol for UBA2 antibody 15347-1-APDownload protocol
IF protocol for UBA2 antibody 15347-1-APDownload protocol
IP protocol for UBA2 antibody 15347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IHC

Cell Death Differ

Inhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury.

Authors - Jing Huang
  • KD Validated