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UBC12 Monoclonal antibody

UBC12 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H8D2

Cat no : 67482-1-Ig

Synonyms

hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, UBC RS2, UBC12, UBE2M

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using UBC12 Monoclonal antibody (67482-1-Ig)

    WB analysis of HEK-293 using 67482-1-Ig

    WB result of UBC12 antibody (67482-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBC12 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using UBC12 Monoclonal antibody (67482-1-Ig)

    WB analysis using 67482-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67482-1-Ig (UBC12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UBC12 Monoclonal antibody (67482-1-Ig)

    IHC staining of human liver cancer using 67482-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UBC12 Monoclonal antibody (67482-1-Ig)

    IHC staining of human liver cancer using 67482-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBC12 Monoclonal antibody (67482-1-Ig)

    IF Staining of HeLa using 67482-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBC12 antibody (67482-1-Ig, Clone: 1H8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, HepG2 cells, L02 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67482-1-Ig targets UBC12 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBC12 fusion protein Ag5984
Full Name ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC058924
Gene Symbol UBE2M
Gene ID (NCBI) 9040
RRIDAB_2882709
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBC12 antibody 67482-1-IgDownload protocol
IHC protocol for UBC12 antibody 67482-1-IgDownload protocol
IF protocol for UBC12 antibody 67482-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Cell Rep

Type I interferon/STAT1 signaling regulates UBE2M-mediated antiviral innate immunity in a negative feedback manner

Authors - Xianghui Kong
View Article