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UBE2G2 Polyclonal antibody

UBE2G2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10722-1-AP

Synonyms

UBC7, UBE2G2, Ubiquitin carrier protein G2, Ubiquitin protein ligase G2

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using UBE2G2 Polyclonal antibody (10722-1-AP)

    WB analysis of Jurkat using 10722-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10722-1-AP (UBE2G2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using UBE2G2 Polyclonal antibody (10722-1-AP)

    WB analysis of K-562 using 10722-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10722-1-AP (UBE2G2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using UBE2G2 Polyclonal antibody (10722-1-AP)

    WB analysis of mouse testis using 10722-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10722-1-AP (UBE2G2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UBE2G2 Polyclonal antibody (10722-1-AP)

    WB analysis of HeLa using 10722-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10722-1-AP (UBE2G2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using UBE2G2 Polyclonal antibody (10722-1-AP)

    IHC staining of human prostate cancer using 10722-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10722-1-AP (UBE2G2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using UBE2G2 Polyclonal antibody (10722-1-AP)

    IHC staining of human prostate cancer using 10722-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10722-1-AP (UBE2G2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, K-562 cells, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10722-1-AP targets UBE2G2 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2G2 fusion protein Ag1082
Full Name ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast)
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 16-19 kDa
GenBank Accession NumberBC008351
Gene Symbol UBE2G2
Gene ID (NCBI) 7327
RRIDAB_2256726
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBE2G2 antibody 10722-1-APDownload protocol
IHC protocol for UBE2G2 antibody 10722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols