MMS2 Polyclonal antibody

MMS2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10689-1-AP

Synonyms

DDVit 1, DDVIT1, EDAF 1, EDPF 1, EDPF1, MMS2, MMS2 homolog, UB2V2, UBE2V2, UEV 2, UEV2, Vitamin D3 inducible protein



Tested Applications

Positive WB detected inHEK-293 cells, A375 cells, HeLa cells, HL-60 cells, human brain tissue, Jurkat cells, K-562 cells, rat liver tissue
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10689-1-AP targets MMS2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMS2 fusion protein Ag1111
Full Name ubiquitin-conjugating enzyme E2 variant 2
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC007051
Gene Symbol UBE2V2
Gene ID (NCBI) 7336
RRIDAB_2211656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MMS2 antibody 10689-1-APDownload protocol
IHC protocol for MMS2 antibody 10689-1-APDownload protocol
IF protocol for MMS2 antibody 10689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF,WB

Transl Stroke Res

MicroRNA-193a-5p Rescues Ischemic Cerebral Injury by Restoring N2-Like Neutrophil Subsets

Authors - Ziping Han
humanWB, IF

Biochim Biophys Acta

Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.

Authors - Fan Chai