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UBF1 Polyclonal antibody

UBF1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20660-1-AP

Synonyms

Autoantigen NOR 90, NOR 90, UBF, UBF 1, UBF1, UBTF, Upstream binding factor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using UBF1 Polyclonal antibody (20660-1-AP)

    WB analysis of HT-1080 using 20660-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using UBF1 Polyclonal antibody (20660-1-AP)

    WB analysis of MCF-7 using 20660-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UBF1 Polyclonal antibody (20660-1-AP)

    WB analysis of HepG2 using 20660-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UBF1 Polyclonal antibody (20660-1-AP)

    IF Staining of HepG2 using 20660-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 20660-1-AP (UBF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHT-1080 cells, HepG2 cells, MCF-7 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20660-1-AP targets UBF1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name upstream binding transcription factor, RNA polymerase I
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberNM_014233
Gene Symbol UBTF
Gene ID (NCBI) 7343
RRIDAB_2878718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBTF, also named as NOR-90 or UBF-1, is a 764 amino acid protein, which may form Homodimer. UBTF recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. UBTF exists two isoform with MV 89 kDa and 85 kDa. (PMID: 1940801)

Protocols

Product Specific Protocols
WB protocol for UBF1 antibody 20660-1-APDownload protocol
IF protocol for UBF1 antibody 20660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tatyana (Verified Customer) (12-04-2023)

Detectable but weak signal in the nucleoli

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human / HeLa cells
UBF1 Antibody Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells (Cat no:20660-1-AP)