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  • KD/KO Validated

UCHL3 Polyclonal antibody

UCHL3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12384-1-AP

Synonyms

Ubiquitin carboxyl terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, UCHL3, UCH-L3



Tested Applications

Positive WB detected inHeLa cells, rat spleen tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12384-1-AP targets UCHL3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCHL3 fusion protein Ag3053
Full Name ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC018125
Gene Symbol UCHL3
Gene ID (NCBI) 7347
RRIDAB_2210915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.

Protocols

Product Specific Protocols
WB protocol for UCHL3 antibody 12384-1-APDownload protocol
IHC protocol for UCHL3 antibody 12384-1-APDownload protocol
IP protocol for UCHL3 antibody 12384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nat Commun

Farrerol directly activates the deubiqutinase UCHL3 to promote DNA repair and reprogramming when mediated by somatic cell nuclear transfer

Authors - Weina Zhang
  • KD Validated
humanWB

Mol Cell

An Interaction Landscape of Ubiquitin Signaling.

Authors - Xiaofei Zhang
humanWB

Nat Commun

Structural basis for specific inhibition of the deubiquitinase UCHL1

Authors - Christian Grethe
  • KD Validated
humanWB

Genes Dev

A phosphorylation-deubiquitination cascade regulates the BRCA2-RAD51 axis in homologous recombination.

Authors - Kuntian Luo
  • KO Validated
humanWB,IHC

Am J Cancer Res

UCHL3 promotes pancreatic cancer progression and chemo-resistance through FOXM1 stabilization.

Authors - Zhiwang Song
  • KD Validated
mouseWB

Sci Rep

Ubiquitin C-terminal hydrolase L1 (UCHL1) regulates post-myocardial infarction cardiac fibrosis through glucose-regulated protein of 78 kDa (GRP78).

Authors - Qian Lei