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Urocortin Polyclonal antibody

Urocortin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19731-1-AP

Synonyms

UCN, UI, UROC, urocortin

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Urocortin Polyclonal antibody (19731-1-AP)

    WB analysis of HeLa using 19731-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Urocortin Polyclonal antibody (19731-1-AP)

    WB analysis of HEK-293 using 19731-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using Urocortin Polyclonal antibody (19731-1-AP)

    WB analysis of human kidney using 19731-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using Urocortin Polyclonal antibody (19731-1-AP)

    WB analysis of human testis using 19731-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human kidney tissue, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19731-1-AP targets Urocortin in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name urocortin
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberNM_003353
Gene Symbol UCN
Gene ID (NCBI) 7349
RRIDAB_10640909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).

Protocols

Product Specific Protocols
WB protocol for Urocortin antibody 19731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols