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UCN Polyclonal antibody

UCN Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC,ELISA

Conjugate

Unconjugated

Cat no : 21951-1-AP

Synonyms

UCN, UI, UROC, urocortin

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human testis tissue using UCN Polyclonal antibody (21951-1-AP)

    IHC staining of human testis using 21951-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21951-1-AP (UCN Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using UCN Polyclonal antibody (21951-1-AP)

    IHC staining of human testis using 21951-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21951-1-AP (UCN Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UCN Polyclonal antibody (21951-1-AP)

    IHC staining of human lung cancer using 21951-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21951-1-AP (UCN Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UCN Polyclonal antibody (21951-1-AP)

    IHC staining of human lung cancer using 21951-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21951-1-AP (UCN Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21951-1-AP is UCN Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCN fusion protein Ag15995
Full Name urocortin
Calculated Molecular Weight 124 aa, 13 kDa
GenBank Accession NumberBC104470
Gene Symbol UCN
Gene ID (NCBI) 7349
RRIDAB_2878953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. . Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ).

Protocols

Product Specific Protocols
IHC protocol for UCN antibody 21951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols