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UGT3A2 Polyclonal antibody

UGT3A2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21366-1-AP

Synonyms

UDPGT 3A2, UGT3A2, UNQ842/PRO1780

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UGT3A2 Polyclonal antibody (21366-1-AP)

    WB analysis using 21366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21366-1-AP (UGT3A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using UGT3A2 Polyclonal antibody (21366-1-AP)

    WB analysis of human testis using 21366-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 21366-1-AP (UGT3A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using UGT3A2 Polyclonal antibody (21366-1-AP)

    IHC staining of mouse liver using 21366-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21366-1-AP (UGT3A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using UGT3A2 Polyclonal antibody (21366-1-AP)

    IHC staining of mouse liver using 21366-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21366-1-AP (UGT3A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMOLT-4 cells, human testis tissue, THP-1 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 21366-1-AP is UGT3A2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UGT3A2 fusion protein Ag15835
Full Name UDP glycosyltransferase 3 family, polypeptide A2
Calculated Molecular Weight 523 aa, 60 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC103925
Gene Symbol UGT3A2
Gene ID (NCBI) 167127
RRIDAB_10888629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UGT3A2 antibody 21366-1-APDownload protocol
IHC protocol for UGT3A2 antibody 21366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Front Mol Biosci

Identification of small extracellular vesicle protein biomarkers for pediatric Ewing Sarcoma

Authors - Soumya M Turaga
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Daniel (Verified Customer) (10-26-2022)

This antibody works but produces high background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa, MT4 cells
UGT3A2 Antibody Western Blot validation (1:500 dilution) in HeLa, MT4 cells (Cat no:21366-1-AP)