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  • KD/KO Validated

UGT8 Polyclonal antibody

UGT8 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17982-1-AP

Synonyms

ceramide galactosyltransferase, Cerebroside synthase, CGT, UDP glycosyltransferase 8, UGT4, UGT8



Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue, mouse cerebellum tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17982-1-AP targets UGT8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UGT8 fusion protein Ag12393
Full Name UDP glycosyltransferase 8
Calculated Molecular Weight 541 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC075069
Gene Symbol UGT8
Gene ID (NCBI) 7368
RRIDAB_2214254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UGT8, also known as galacotosylceramide synthase or CGT (ceramide galactosyltransferase), is a key enzyme for galacotosylceramide (GalCer) biosynthesis. It is an ER transmembrane protein and has limited tissue distribution, with predominance in Schwann cells, oligodendrocytes, kidneys, testes, and intestine. UGT8 is highly enriched in myelin in the central nervous system. Its expression levels are strongly associated with histological typing in human oligodendrogliomas and astrocytomas; can be used as molecular marker to distinguish these tumors. Higher UGT8 levels had also been linked to metastatic properties of cancer cells. This antibody specifically recognizes the endogenous UGT8.

Protocols

Product Specific Protocols
WB protocol for UGT8 antibody 17982-1-APDownload protocol
IHC protocol for UGT8 antibody 17982-1-APDownload protocol
IP protocol for UGT8 antibody 17982-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Exp Med

Inhibition of UGT8 suppresses basal-like breast cancer progression by attenuating sulfatide-αVβ5 axis.

Authors - Qianhua Cao
  • KD Validated
mouse,humanWB,IHC

Mol Cell Proteomics

Metabolic Enzyme Alterations and Astrocyte Dysfunction in a Murine Model of Alexander Disease With Severe Reactive Gliosis.

Authors - Michael R Heaven
humanWB

Mol Pharmacol

Circular RNAs of UDP-Glycosyltransferase (UGT) Genes Expand the Complexity and Diversity of the UGT Transcriptome.

Authors - Dong Gui Hu
humanIHC

Biosci Rep

Uronic acid metabolic process-related gene expression-based signature predicts overall survival of glioma.

Authors - Yuemei Feng
rat, humanWB,IHC

Cancer Chemother Pharmacol

Evaluation of resveratrol sensitivities and metabolic patterns in human and rat glioblastoma cells.

Authors - Sun Zheng Z
ratWB, IHC

Neurotherapeutics

Diffusion Efficiency and Bioavailability of Resveratrol Administered to Rat Brain by Different Routes: Therapeutic Implications.

Authors - Xiao-Hong Shu