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UNC5A-Specific Polyclonal antibody

UNC5A-Specific Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC,ELISA

Conjugate

Unconjugated

Cat no : 20239-1-AP

Synonyms

KIAA1976, Netrin receptor UNC5A, Protein unc 5 homolog 1, Protein unc 5 homolog A, unc 5 homolog A (C. elegans), UNC5A, UNC5H1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using UNC5A-Specific Polyclonal antibody (20239-1-AP)

    WB analysis of mouse brain using 20239-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20239-1-AP (UNC5A-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using UNC5A-Specific Polyclonal antibody (20239-1-AP)

    WB analysis of mouse brain using 20239-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20239-1-AP (UNC5A-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using UNC5A-Specific Polyclonal antibody (20239-1-AP)

    FC experiment of MCF-7 using 20239-1-AP

    1X10^6 MCF-7 cells were stained with 0.2ug UNC5A-Specific antibody (20239-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inmouse brain tissue
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20239-1-AP targets UNC5A-Specific in WB, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name unc-5 homolog A (C. elegans)
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 103 kDa
GenBank Accession NumberNM_133369
Gene Symbol UNC5A
Gene ID (NCBI) 90249
RRIDAB_10695050
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNC5A, also named as KIAA1976 and UNC5H1, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5A. It has no cross reaction to UNC5B, UNC5C and UNC5D.

Protocols

Product Specific Protocols
WB protocol for UNC5A-Specific antibody 20239-1-APDownload protocol
FC protocol for UNC5A-Specific antibody 20239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols