- Featured Product
- KD/KO Validated
UPF1 Polyclonal antibody
UPF1 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, RIP, IP, IF, ELISA
Conjugate
Unconjugated
Cat no : 23379-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Raji cells, SH-SY5Y cells |
| Positive IP detected in | Raji cells |
| Positive IF detected in | HepG2 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IF | See 2 publications below |
| RIP | See 1 publications below |
Product Information
23379-1-AP targets UPF1 in WB, RIP, IP, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UPF1 fusion protein Ag16195 |
| Full Name | UPF1 regulator of nonsense transcripts homolog (yeast) |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 123-130 kDa |
| GenBank Accession Number | BC039817 |
| Gene Symbol | UPF1 |
| Gene ID (NCBI) | 5976 |
| RRID | AB_11232421 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UPF1 antibody 23379-1-AP | Download protocol |
| IF protocol for UPF1 antibody 23379-1-AP | Download protocol |
| IP protocol for UPF1 antibody 23379-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1 | ||
Breast Cancer Res Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1. | ||
Bioengineered The biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells. | ||
J Exp Clin Cancer Res PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α. | ||
Cell Death Dis PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay. | ||
Cell Rep UPF1 reduces C9orf72 HRE-induced neurotoxicity in the absence of nonsense-mediated decay dysfunction.
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