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UPK1A Polyclonal antibody

UPK1A Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25275-1-AP

Synonyms

Tetraspanin 21, Tspan 21, TSPAN21, UP1A, UPIA, UPK1A, UPKA, uroplakin 1A, Uroplakin Ia

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse bladder tissue using UPK1A Polyclonal antibody (25275-1-AP)

    IP experiment of mouse bladder using 25275-1-AP

    IP result of anti-UPK1A (IP:25275-1-AP, 4ug; Detection:25275-1-AP 1:300) with mouse bladder tissue lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human bladder tissue using UPK1A Polyclonal antibody (25275-1-AP)

    IHC staining of human bladder using 25275-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:5000 (under 10x lens)..

  • Immunohistochemistry (IHC) staining of human bladder tissue using UPK1A Polyclonal antibody (25275-1-AP)

    IHC staining of human bladder using 25275-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:5000 (under 40x lens)..

  • Immunohistochemistry (IHC) staining of rat bladder tissue using UPK1A Polyclonal antibody (25275-1-AP)

    IHC staining of rat bladder using 25275-1-AP

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat bladder tissue using UPK1A Polyclonal antibody (25275-1-AP)

    IHC staining of rat bladder using 25275-1-AP

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using UPK1A Polyclonal antibody (25275-1-AP)

    IHC staining of mouse bladder using 25275-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using UPK1A Polyclonal antibody (25275-1-AP)

    IHC staining of mouse bladder using 25275-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IP detected inmouse bladder tissue
Positive IHC detected inhuman bladder tissue, rat bladder tissue, mouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25275-1-AP is UPK1A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UPK1A fusion protein Ag17356
Full Name uroplakin 1A
Calculated Molecular Weight 258 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC107098
Gene Symbol UPK1A
Gene ID (NCBI) 11045
RRIDAB_2880000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-1a (UPK1A) belongs to the tetraspanin (TM4SF) family. UPK1A plays an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane.

Protocols

Product Specific Protocols
IHC protocol for UPK1A antibody 25275-1-APDownload protocol
IP protocol for UPK1A antibody 25275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Stem Cells Int

Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.

Authors - Cong Li
View Article
humanIHC

Int J Urol

Differential effects of adipose tissue stromal cells on the apoptosis, growth and invasion of bladder urothelial carcinoma between the superficial and invasive types.

Authors - Maki Kawasaki-Nanri
View Article