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  • Featured Product
  • KD/KO Validated

UQCRC2 Polyclonal antibody

UQCRC2 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14742-1-AP

Synonyms

Complex III subunit 2, Core protein II, QCR2, UQCR2, UQCRC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRC2 Polyclonal antibody (14742-1-AP)

    WB analysis using 14742-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14742-1-AP (UQCRC2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using UQCRC2 Polyclonal antibody (14742-1-AP)

    WB analysis of human colon using 14742-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 14742-1-AP (UQCRC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    IP experiment of HeLa using 14742-1-AP

    IP result of anti-UQCRC2 (IP:14742-1-AP, 4ug; Detection:14742-1-AP 1:40000) with HeLa cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UQCRC2 Polyclonal antibody (14742-1-AP)

    IHC staining of human colon cancer using 14742-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14742-1-AP (UQCRC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UQCRC2 Polyclonal antibody (14742-1-AP)

    IHC staining of human colon cancer using 14742-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14742-1-AP (UQCRC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    IF Staining of HepG2 using 14742-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14742-1-AP (UQCRC2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L); Phalloidin stains F-actin (Red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    IF Staining of HepG2 using 14742-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14742-1-AP (UQCRC2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    FC experiment of HepG2 using 14742-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug UQCRC2 antibody (14742-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inmouse brain tissue, human colon tissue, mouse heart tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14742-1-AP targets UQCRC2 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UQCRC2 fusion protein Ag6432
Full Name ubiquinol-cytochrome c reductase core protein II
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000484
Gene Symbol UQCRC2
Gene ID (NCBI) 7385
RRIDAB_2241442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.

Protocols

Product Specific Protocols
WB protocol for UQCRC2 antibody 14742-1-APDownload protocol
IHC protocol for UQCRC2 antibody 14742-1-APDownload protocol
IF protocol for UQCRC2 antibody 14742-1-APDownload protocol
IP protocol for UQCRC2 antibody 14742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
sheepWB

Nature

Structure and assembly of the mammalian mitochondrial supercomplex CIII2CIV.

Authors - Irene Vercellino
View Article
mouseWB

Nature

Mechanism of super-assembly of respiratory complexes III and IV.

Authors - Sara Cogliati
View Article
humanWB

Cell

Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.

Authors - Wen Yang
View Article
mouseWB

Cell Metab

Regulation of Mother-to-Offspring Transmission of mtDNA Heteroplasmy.

Authors - Ana Latorre-Pellicer
View Article
mouseWB

Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi
View Article
mouseWB

Sci Adv

Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Qpool.

Authors - Enrique Calvo
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate UQCRC2 works well, both with ECL detection and fluorescent secondary antibodies it gives a nice strong band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: K562 and KCL22