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URG4 Polyclonal antibody

URG4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11998-1-AP

Synonyms

KIAA1507, up regulated gene 4, URG4, URGCP



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman gliomas tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11998-1-AP targets URG4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen URG4 fusion protein Ag2630
Full Name up-regulated gene 4
Calculated Molecular Weight 922 aa, 104 kDa
Observed Molecular Weight 104 kDa
GenBank Accession NumberBC018426
Gene Symbol URG4
Gene ID (NCBI) 55665
RRIDAB_1072845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.

Protocols

Product Specific Protocols
WB protocol for URG4 antibody 11998-1-APDownload protocol
IHC protocol for URG4 antibody 11998-1-APDownload protocol
IF protocol for URG4 antibody 11998-1-APDownload protocol
IP protocol for URG4 antibody 11998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bone

Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway

Authors - Yuanxi Leng
humanIHC,WB

J Orthop Surg Res

URG4 mediates cell proliferation and cell cycle in osteosarcoma via GSK3β/β-catenin/cyclin D1 signaling pathway.

Authors - Yayun Liu
  • KD Validated