URG4 Monoclonal antibody
URG4 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2D5F12
Cat no : 67144-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | SGC-7901 cells, MKN-45 cells, HepG2 cells, HuH-7 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, human nasopharyngeal carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67144-1-Ig targets URG4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | URG4 fusion protein Ag28635 |
| Full Name | up-regulated gene 4 |
| Calculated Molecular Weight | 922 aa, 104 kDa |
| Observed Molecular Weight | 104 kDa |
| GenBank Accession Number | BC018426 |
| Gene Symbol | URG4 |
| Gene ID (NCBI) | 55665 |
| RRID | AB_2882443 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for URG4 antibody 67144-1-Ig | Download protocol |
| IHC protocol for URG4 antibody 67144-1-Ig | Download protocol |
| IF protocol for URG4 antibody 67144-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
