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Vitamin D binding protein Polyclonal antibody

Vitamin D binding protein Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16922-1-AP

Synonyms

DBP, DBP/GC, GC, Gc globulin, Group specific component, VDB, VDBG, VDBP, Vitamin D binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Vitamin D binding protein Polyclonal antibody (16922-1-AP)

    WB analysis of human plasma using 16922-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat eye tissue using Vitamin D binding protein Polyclonal antibody (16922-1-AP)

    WB analysis of rat eye using 16922-1-AP

    rat eye tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (Vitamin D binding protein antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using Vitamin D binding protein Polyclonal antibody (16922-1-AP)

    WB analysis of mouse testis using 16922-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using Vitamin D binding protein Polyclonal antibody (16922-1-AP)

    WB analysis of mouse eye using 16922-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using Vitamin D binding protein Polyclonal antibody (16922-1-AP)

    WB analysis of PC-3 using 16922-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using Vitamin D binding protein Polyclonal antibody (16922-1-AP)

    WB analysis of mouse eye using 16922-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Vitamin D binding protein Polyclonal antibody (16922-1-AP)

    IHC staining of human liver using 16922-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16922-1-AP (Vitamin D binding protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Vitamin D binding protein Polyclonal antibody (16922-1-AP)

    IHC staining of human liver using 16922-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16922-1-AP (Vitamin D binding protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman blood tissue, human blood, rat eye tissue, mouse eye tissue, PC-3 cells, human plasma, mouse testis tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16922-1-AP targets Vitamin D binding protein in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vitamin D binding protein fusion protein Ag10286
Full Name group-specific component (vitamin D binding protein)
Calculated Molecular Weight 474 aa, 53 kDa
Observed Molecular Weight 52-58 kDa
GenBank Accession NumberBC057228
Gene Symbol GC
Gene ID (NCBI) 2638
RRIDAB_10597098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

Protocols

Product Specific Protocols
WB protocol for Vitamin D binding protein antibody 16922-1-APDownload protocol
IHC protocol for Vitamin D binding protein antibody 16922-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Ther Methods Clin Dev

Lentiviral vector mediated gene therapy for type I Dent disease ameliorates Dent disease-like phenotypes for three months in ClC-5 null mice

Authors - Manish Kumar Yadav
View Article
ratWB

Sci Rep

PMID: 31792309

Authors - Nela Pavlíková
View Article

Proteomics

Screening for potential serum biomarkers in rat mesangial proliferative nephritis.

Authors - Yang Lu
View Article
ratWB,IHC

Mol Med Rep

Proteomics of acute heart failure in a rat post-myocardial infarction model.

Authors - Yichen Guo
View Article
mouseWB

Pharmaceutics

Evaluation of In Vitro and In Vivo Antiviral Activities of Vitamin D for SARS-CoV-2 and Variants

Authors - Chee-Keng Mok
View Article
mouseIHC

Int Immunopharmacol

Vitamin D attenuates DNCB-induced atopic dermatitis-like skin lesions by inhibiting immune response and restoring skin barrier function

Authors - Renwei Lu
View Article