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VEZT Polyclonal antibody

VEZT Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17684-1-AP

Synonyms

VEZATIN, VEZT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VEZT Polyclonal antibody (17684-1-AP)

    WB analysis using 17684-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using VEZT Polyclonal antibody (17684-1-AP)

    WB analysis of human brain using 17684-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using VEZT Polyclonal antibody (17684-1-AP)

    WB analysis of mouse brain using 17684-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using VEZT Polyclonal antibody (17684-1-AP)

    IP experiment of mouse brain using 17684-1-AP

    IP result of anti-VEZT (IP:17684-1-AP, 4ug; Detection:17684-1-AP 1:500) with mouse brain tissue lysate 4400ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VEZT Polyclonal antibody (17684-1-AP)

    IHC staining of mouse brain using 17684-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VEZT Polyclonal antibody (17684-1-AP)

    IHC staining of mouse brain using 17684-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse eye tissue, human brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17684-1-AP targets VEZT in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VEZT fusion protein Ag11786
Full Name vezatin, adherens junctions transmembrane protein
Calculated Molecular Weight 731 aa, 83 kDa
Observed Molecular Weight 89 kDa, 125 kDa
GenBank Accession NumberBC064939
Gene Symbol VEZT
Gene ID (NCBI) 55591
RRIDAB_2213139
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vezatin (VEZT) is an adherens junctions transmembrane protein which identified as a putative tumor suppressor. In case of Listeria infection, it promotes bacterial internalization by participating in myosin VIIa recruitment to the entry site. There're two isoforms with calculated MW 89 kDa and 65 kDa. With modification or glycosylation, the MW in Western blot detection will be presented as ~125 kDa and ~89 kDa (PMID: 11080149) or 65-71 kDa and 88-100 kDa (PMID: 20049712). There're some isoforms with MW less than 70 kDa.

Protocols

Product Specific Protocols
WB protocol for VEZT antibody 17684-1-APDownload protocol
IHC protocol for VEZT antibody 17684-1-APDownload protocol
IP protocol for VEZT antibody 17684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols