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  • Featured Product
  • KD/KO Validated

VPS37B Polyclonal antibody

VPS37B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15653-1-AP

Synonyms

ESCRT I complex subunit VPS37B, hVps37B, VPS37B

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using VPS37B Polyclonal antibody (15653-1-AP)

    WB analysis of HeLa using 15653-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15653-1-AP (VPS37B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using VPS37B Polyclonal antibody (15653-1-AP)

    WB analysis of COLO 320 using 15653-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15653-1-AP (VPS37B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15653-1-AP targets VPS37B in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS37B fusion protein Ag8256
Full Name vacuolar protein sorting 37 homolog B (S. cerevisiae)
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC005882
Gene Symbol VPS37B
Gene ID (NCBI) 79720
RRIDAB_2878161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VPS37B antibody 15653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

Removal of hypersignaling endosomes by simaphagy

Authors - Simona M Migliano
  • KD Validated
View Article
mouseWB

Autophagy Rep

Upregulation of the ESCRT pathway and multivesicular bodies accelerates degradation of proteins associated with neurodegeneration

Authors - Ron Benyair
  • KD Validated
View Article
humanWB

EMBO J

Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs

Authors - Ruheena Javed
  • KO Validated
View Article
humanWB

J Cell Sci

UMAD1 contributes to ESCRT-III dynamic subunit turnover during cytokinetic abscission

Authors - James Glover
View Article
humanWB

Bioorg Chem

Chemoproteomics reveals arctigenin as a phagophore-closure blocker via targeting ESCRT-I subunit VPS28

Authors - Jinyan Han
View Article