VSX2 Polyclonal antibody

VSX2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25825-1-AP

Synonyms

CHX10, Homeobox protein CHX10, HOX10, MCOP2, MCOPCB3, RET1, visual system homeobox 2, VSX2



Tested Applications

Positive WB detected inmouse eye tissue
Positive IHC detected inhuman retinoblastoma tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

25825-1-AP targets VSX2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VSX2 fusion protein Ag23035
Full Name visual system homeobox 2
Calculated Molecular Weight 361 aa, 39 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC128153
Gene Symbol VSX2
Gene ID (NCBI) 338917
RRIDAB_2880257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VSX2 antibody 25825-1-APDownload protocol
IHC protocol for VSX2 antibody 25825-1-APDownload protocol
IF protocol for VSX2 antibody 25825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Human brain organoids assemble functionally integrated bilateral optic vesicles.

Authors - Elke Gabriel
humanIF

Nat Protoc

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia

Authors - Elke Gabriel

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alessandro (Verified Customer) (11-06-2022)

no unspecific staining, great outcome

  • Applications: Immunofluorescence