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- KD/KO Validated
WASP Polyclonal antibody
WASP Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10987-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, HL-60 cells, K-562 cells, mouse spleen tissue |
| Positive IP detected in | Raji cells, rat spleen tissue |
| Positive IHC detected in | human spleen tissue, human lymphoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
10987-1-AP targets WASP in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WASP fusion protein Ag1444 |
| Full Name | Wiskott-Aldrich syndrome (eczema-thrombocytopenia) |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 55-62 kDa |
| GenBank Accession Number | BC012738 |
| Gene Symbol | WAS |
| Gene ID (NCBI) | 7454 |
| RRID | AB_2304546 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WASP antibody 10987-1-AP | Download protocol |
| IHC protocol for WASP antibody 10987-1-AP | Download protocol |
| IP protocol for WASP antibody 10987-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Allergy Clin Immunol Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation. | ||
Nat Commun WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage. | ||
J Exp Clin Cancer Res miR-182 suppresses invadopodia formation and metastasis in non-small cell lung cancer by targeting cortactin gene.
| ||
J Exp Clin Cancer Res CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Seong-Su (Verified Customer) (09-17-2020) | I checked nine WASp Abs from 4 different companies for ELISA and EMSA super-shift. Among them this proteintech WASp Ab showed wonderful result for ELISA as well as was the only Ab that showed shifted bands of WASp binding complex.My colleague used this Ab for western and got a beautiful result.
|
FH Nisreen (Verified Customer) (11-07-2017) | The antibody are clean and nice.
|
