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  • Featured Product
  • KD/KO Validated

WIPI1 Polyclonal antibody

WIPI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25204-1-AP

Synonyms

ATG18, Atg18 protein homolog, WIPI 1, WIPI 49 kDa, WIPI1, WIPI49

Validation Data Gallery

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  • Western Blot (WB) analysis of SW 1990 cells using WIPI1 Polyclonal antibody (25204-1-AP)

    WB analysis of SW 1990 using 25204-1-AP

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW 1990 cells using WIPI1 Polyclonal antibody (25204-1-AP)

    WB analysis of SW 1990 using 25204-1-AP

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using WIPI1 Polyclonal antibody (25204-1-AP)

    WB analysis of NIH/3T3 using 25204-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using WIPI1 Polyclonal antibody (25204-1-AP)

    WB analysis of HEK-293 using 25204-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSW 1990 cells, HEK-293 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25204-1-AP is WIPI1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WIPI1 fusion protein Ag18461
Full Name WD repeat domain, phosphoinositide interacting 1
Calculated Molecular Weight 446 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC039867
Gene Symbol WIPI1
Gene ID (NCBI) 55062
RRIDAB_2879958
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for WIPI1 antibody 25204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Oral Oncol

WIPI-1 inhibits metastasis and tumour growth via the WIPI-1-TRIM21 axis and MYC regulation in nasopharyngeal carcinoma.

Authors - Yin Zhao
  • KD Validated
View Article