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  • KD/KO Validated

WNT1 Polyclonal antibody

WNT1 Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 27935-1-AP

Synonyms

INT1, Proto oncogene Int 1 homolog, Proto oncogene Wnt 1, WNT1



Tested Applications

Positive WB detected in3T3-L1 cells, HepG2 cells, NIH/3T3 cells, Jurkat cells
Positive IF detected inNIH/3T3 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27935-1-AP targets WNT1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT1 fusion protein Ag27380
Full Name wingless-type MMTV integration site family, member 1
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC074799
Gene Symbol WNT1
Gene ID (NCBI) 7471
RRIDAB_2881013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for WNT1 antibody 27935-1-APDownload protocol
IF protocol for WNT1 antibody 27935-1-APDownload protocol
FC protocol for WNT1 antibody 27935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bioengineered

MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1)

Authors - Guang-Lin Song
humanWB

Neural Regen Res

Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke

Authors - Jianfeng Wang
humanWB

Aging (Albany NY)

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.

Authors - Liansheng Sha
mouseWB

Microb Biotechnol

Combination of an engineered Lactococcus lactis expressing CXCL12 with light-emitting diode yellow light as a treatment for scalded skin in mice.

Authors - Xiaoxiao Zhao
humanWB,IHC

Front Oncol

Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6.

Authors - Hanqing Wang
mouseWB

Transplantation

Agmatine Attenuates Liver Ischemia Reperfusion Injury by Activating Wnt/β-catenin Signaling in Mice.

Authors - Zhenyi Han