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XRN2 Monoclonal antibody

XRN2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2C3E3

Cat no : 66852-1-Ig

Synonyms

5 3 exoribonuclease 2, 5' 3' exoribonuclease 2, DHM1 like protein, DHP protein, XRN2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XRN2 Monoclonal antibody (66852-1-Ig)

    WB analysis using 66852-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66852-1-Ig (XRN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using XRN2 Monoclonal antibody (66852-1-Ig)

    WB analysis of HEK-293 using 66852-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66852-1-Ig (XRN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using XRN2 Monoclonal antibody (66852-1-Ig)

    WB analysis of COLO 320 using 66852-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66852-1-Ig (XRN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRN2 Monoclonal antibody (66852-1-Ig)

    IHC staining of human breast cancer using 66852-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66852-1-Ig (XRN2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRN2 Monoclonal antibody (66852-1-Ig)

    IHC staining of human breast cancer using 66852-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66852-1-Ig (XRN2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using XRN2 Monoclonal antibody (66852-1-Ig)

    IF Staining of HepG2 using 66852-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66852-1-Ig (XRN2 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using XRN2 Monoclonal antibody (66852-1-Ig)

    FC experiment of HepG2 using 66852-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XRN2 (66852-1-Ig, Clone:2C3E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHT-29 cells, HEK-293 cells, COLO 320 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66852-1-Ig targets XRN2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen XRN2 fusion protein Ag27927
Full Name 5'-3' exoribonuclease 2
Calculated Molecular Weight 104 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberBC006417
Gene Symbol XRN2
Gene ID (NCBI) 22803
RRIDAB_2882192
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.

Protocols

Product Specific Protocols
WB protocol for XRN2 antibody 66852-1-IgDownload protocol
IHC protocol for XRN2 antibody 66852-1-IgDownload protocol
IF protocol for XRN2 antibody 66852-1-IgDownload protocol
FC protocol for XRN2 antibody 66852-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Physiol Cell Physiol

Hydrogen sulfide rescues high glucose-induced migration dysfunction in HUVECs by upregulating miR-126-3p.

Authors - Wen-Long Xue
View Article
humanWB

Cancer Res

NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer

Authors - Ruihui Xie
View Article