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YIPF4 Polyclonal antibody

YIPF4 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15473-1-AP

Synonyms

FinGER4, Nbla11189, Protein YIPF4, Yip1 domain family, member 4, YIP1 family member 4, YIPF4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YIPF4 Polyclonal antibody (15473-1-AP)

    WB analysis using 15473-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using YIPF4 Polyclonal antibody (15473-1-PBS)

    WB analysis of HeLa using 15473-1-PBS

    HeLa cells were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using YIPF4 Polyclonal antibody (15473-1-AP)

    WB analysis using 15473-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using YIPF4 Polyclonal antibody (15473-1-PBS)

    WB analysis of Jurkat using 15473-1-PBS

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using YIPF4 Polyclonal antibody (15473-1-AP)

    IHC staining of human endometrial cancer using 15473-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using YIPF4 Polyclonal antibody (15473-1-AP)

    IHC staining of human endometrial cancer using 15473-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YIPF4 Polyclonal antibody (15473-1-AP)

    IF Staining of HeLa using 15473-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15473-1-AP (YIPF4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: CL594-phalloidin staining of F-actin.

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, HaCaT cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15473-1-AP targets YIPF4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YIPF4 fusion protein Ag7777
Full Name Yip1 domain family, member 4
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC004875
Gene Symbol YIPF4
Gene ID (NCBI) 84272
RRIDAB_2217206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for YIPF4 antibody 15473-1-APDownload protocol
IHC protocol for YIPF4 antibody 15473-1-APDownload protocol
IF protocol for YIPF4 antibody 15473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols