ZBTB7B Polyclonal antibody

ZBTB7B Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11341-1-AP

Synonyms

c Krox, hcKrox, THPOK, ZBTB15, ZBTB7B, Zfp 67, ZFP67, Zinc finger protein 67 homolog, Zinc finger protein Th POK, ZNF857B



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF detected inHela cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11341-1-AP targets ZBTB7B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, hamster, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZBTB7B fusion protein Ag1888
Full Name zinc finger and BTB domain containing 7B
Calculated Molecular Weight 539 aa, 58 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC012070
Gene Symbol ZBTB7B
Gene ID (NCBI) 51043
RRIDAB_2217245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZBTB7B belongs to a large family of transcription factors, generally acting as repressors, characterized by a carboxy-terminal DNA binding domain made of multiple zinc fingers (four in Thpok) and an amino-terminal BTB-POZ domain that mediates homo- (and possibly hetero-) dimerization [PMID: 17084908]. Zbtb7b is up-regulated by MHC-II-restricted thymocytes during their CD4 differentiation and is a major determinant of CD4 lineage choice. Two properties of ZBTB7B deserve attention. First, although Thpok is expressed in a wide variety of cells, its expression in the thymus is highly lineage-specific : CD4 SP thymocytes (and all CD4 T cells) express Thpok, whereas DP and CD8 SP thymocytes do notSecond, both loss- and gain-of-function experiments indicate that Thpok affects lineage choice but not positive selection [PMID: 15729333, 10550051].

Protocols

Product Specific Protocols
WB protocol for ZBTB7B antibody 11341-1-APDownload protocol
IF protocol for ZBTB7B antibody 11341-1-APDownload protocol
IP protocol for ZBTB7B antibody 11341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
hamsterWB

Acta Biomater

LAP2β transmits force to upregulate genes via chromatin domain stretching but not compression

Authors - Jian Sun
humanWB,IHC

J Transl Med

Epigenetic DNA methylation of Zbtb7b regulates the population of double-positive CD4+CD8+ T cells in ulcerative colitis.

Authors - Hao-Ming Xu
bovineWB,IP

J Agric Food Chem

Acylated Ghrelin Activates PI3K/mTOR Signaling Pathway by Promoting ThPOK Acetylation to Promote Milk Fat Synthesis in Bovine Mammary Epithelial Cells

Authors - Jiaxin Wang