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ZIC3 Polyclonal antibody

ZIC3 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 21717-1-AP

Synonyms

HTX, HTX1, ZIC3, Zinc finger protein ZIC 3, ZNF203

Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of Neuro-2a using 21717-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of SH-SY5Y using 21717-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of SH-SY5Y using 21717-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of mouse cerebellum using 21717-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of SH-SY5Y using 21717-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of Y79 using 21717-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of mouse cerebellum using 21717-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNeuro-2a cells, mouse cerebellum tissue, SH-SY5Y cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21717-1-AP is ZIC3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZIC3 fusion protein Ag16354
Full Name Zic family member 3 (odd-paired homolog, Drosophila)
Calculated Molecular Weight 467 aa, 51 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC113395
Gene Symbol ZIC3
Gene ID (NCBI) 7547
RRIDAB_2878910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZIC3 antibody 21717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other