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  • Featured Product
  • KD/KO Validated

ZNF395 Polyclonal antibody

ZNF395 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11759-1-AP

Synonyms

HD regulating factor 2, HDBP 2, HDBP2, HDRF 2, Papillomavirus binding factor, PBF, PRF 1, PRF1, Si 1 8 14, zinc finger protein 395, ZNF395

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ZNF395 Polyclonal antibody (11759-1-AP)

    WB analysis of human brain using 11759-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZNF395 Polyclonal antibody (11759-1-AP)

    WB analysis of HepG2 using 11759-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF395 Polyclonal antibody (11759-1-AP)

    WB analysis of HeLa using 11759-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ZNF395 Polyclonal antibody (11759-1-AP)

    IHC staining of human pancreas cancer using 11759-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11759-1-AP (ZNF395 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF395 Polyclonal antibody (11759-1-AP)

    IF Staining of HepG2 using 11759-1-AP

    Immunofluorescent analysis of HepG2 cells using 11759-1-AP (ZNF395 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11759-1-AP targets ZNF395 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF395 fusion protein Ag2355
Full Name zinc finger protein 395
Calculated Molecular Weight 513 aa, 55 kDa
Observed Molecular Weight 62 kDa, 72 kDa
GenBank Accession NumberBC001237
Gene Symbol ZNF395
Gene ID (NCBI) 55893
RRIDAB_2218065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF395 is a transcription factors binding to a 7bp GC rich sequence which resides in triplicate at intervals of 13bp within and proximal to the -20bp direct repeat sequences of the Htt promoter. It can bind to regulatory regions in papillomaviruses (PV) and subsequently called the protein papillomavirus binding factor (PBF) [PMID: 11853404]. It contains conserved regions CR1, CR2 and CR3, a domain rich in serines and prolines and have the potential to form a zinc-finger structure, and the C-terminal CR3 is responsible for DNA-binding [PMID: 17897615].

Protocols

Product Specific Protocols
WB protocol for ZNF395 antibody 11759-1-APDownload protocol
IHC protocol for ZNF395 antibody 11759-1-APDownload protocol
IF protocol for ZNF395 antibody 11759-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Onco Targets Ther

Dysregulation of Zinc Finger Protein 395 Contributes to the Pathogenesis of Chondrosarcoma.

Authors - Changbao Chen
View Article
humanWB,IHC

Pathobiology

Downregulation of ZNF395 Drives Progression of Pancreatic Ductal Adenocarcinoma through Enhancement of Growth Potential.

Authors - Shusaku Kurogi
View Article
humanWB

J Exp Clin Cancer Res

HnRNP A1 - mediated alternative splicing of CCDC50 contributes to cancer progression of clear cell renal cell carcinoma via ZNF395.

Authors - Guoliang Sun
  • KD Validated
View Article