CoraLite®594-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for IF, WB

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, plant, Zebrafish, canine

Applications

WB, IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

7D2C10

Cat no : CL594-60008

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells
Positive IF detected inHeLa cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60008 targets Beta Actin in WB, IF applications and shows reactivity with human, mouse, rat, plant, Zebrafish, canine samples.

Tested Reactivity human, mouse, rat, plant, Zebrafish, canine
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Beta Actin fusion protein Ag0297
Full Name actin, beta
Calculated Molecular Weight 375 aa, 42 kDa
GenBank Accession NumberBC002409
Gene Symbol ACTB
Gene ID (NCBI) 60
RRIDAB_2883409
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody is conjugated with CL594,Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
WB protocol for CL594 Beta Actin antibody CL594-60008Download protocol
IF protocol for CL594 Beta Actin antibody CL594-60008Download protocol
Standard Protocols
Click here to view our Standard Protocols