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c-SRC Polyclonal antibody

c-SRC Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, hamster, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25978-1-AP

Synonyms

ASV, c SRC, c-SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using c-SRC Polyclonal antibody (25978-1-AP)

    WB analysis of A431 using 25978-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using c-SRC Polyclonal antibody (25978-1-AP)

    WB analysis of mouse brain using 25978-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using c-SRC Polyclonal antibody (25978-1-AP)

    WB analysis of Jurkat using 25978-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using c-SRC Polyclonal antibody (25978-1-AP)

    WB analysis of COLO 320 using 25978-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of CHO cells using c-SRC Polyclonal antibody (25978-1-AP)

    WB analysis of CHO using 25978-1-AP

    CHO cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using c-SRC Polyclonal antibody (25978-1-AP)

    IHC staining of human breast cancer using 25978-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25978-1-AP (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using c-SRC Polyclonal antibody (25978-1-AP)

    IHC staining of human breast cancer using 25978-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25978-1-AP (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA431 cells, CHO cells, COLO 320 cells, Jurkat cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

25978-1-AP targets c-SRC in WB, IHC, ELISA applications and shows reactivity with mouse, hamster, human samples.

Tested Reactivity mouse, hamster, human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-SRC fusion protein Ag22415
Full Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC011566
Gene Symbol SRC
Gene ID (NCBI) 6714
RRIDAB_2880316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

Protocols

Product Specific Protocols
WB protocol for c-SRC antibody 25978-1-APDownload protocol
IHC protocol for c-SRC antibody 25978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci

Design and screening of a novel neuropilin-1 targeted penetrating peptide for anti-angiogenic therapy in glioma.

Authors - Lin Zhao
View Article
humanWB

Oncotarget

Targeting claudin-4 enhances CDDP-chemosensitivity in gastric cancer.

Authors - Yukiko Nishiguchi
View Article