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hIST1 Monoclonal antibody

hIST1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E12A7

Cat no : 66989-1-Ig

Synonyms

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis using 66989-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis of HSC-T6 using 66989-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis of NIH/3T3 using 66989-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis using 66989-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis of mouse liver using 66989-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using hIST1 Monoclonal antibody (66989-1-Ig)

    IHC staining of human lung cancer using 66989-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using hIST1 Monoclonal antibody (66989-1-Ig)

    IHC staining of human lung cancer using 66989-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using hIST1 Monoclonal antibody (66989-1-Ig)

    IF Staining of HeLa using 66989-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using hIST1 antibody (66989-1-Ig, Clone: 1E12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, mouse liver tissue, HEK-293 cells, MCF-7 cells, Jurkat cells, A549 cells, HepG2 cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66989-1-Ig targets hIST1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen hIST1 fusion protein Ag28364
Full Name KIAA0174
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC000430
Gene Symbol KIAA0174
Gene ID (NCBI) 9798
RRIDAB_2882306
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.

Protocols

Product Specific Protocols
WB protocol for hIST1 antibody 66989-1-IgDownload protocol
IHC protocol for hIST1 antibody 66989-1-IgDownload protocol
IF protocol for hIST1 antibody 66989-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

J Cell Biol

Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters

Authors - Femmy C Stempels
View Article