p504S,AMACR Monoclonal antibody

p504S,AMACR Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2H9B5

Cat no : 60240-1-Ig

Synonyms

2 methylacyl CoA racemase, alpha methylacyl CoA racemase, AMACR, CBAS4, p504S, p504S,AMACR, RACE



Tested Applications

Positive WB detected inHepG2 cells, PC-3 cells, HEK-293 cells, HeLa cells, LNCaP cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60240-1-Ig targets p504S,AMACR in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen p504S,AMACR fusion protein Ag8720
Full Name alpha-methylacyl-CoA racemase
Calculated Molecular Weight 382 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC009471
Gene Symbol AMACR
Gene ID (NCBI) 23600
RRIDAB_2881363
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

Protocols

Product Specific Protocols
WB protocol for p504S,AMACR antibody 60240-1-IgDownload protocol
FC protocol for p504S,AMACR antibody 60240-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols