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phospho-RCC1 (Ser11) Monoclonal antibody

phospho-RCC1(Ser11) Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse

Applications

ELISA

Conjugate

Unconjugated

CloneNo.

5G9D8

Cat no : 60089-1-Ig

Synonyms

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human testis tissue using Phospho-RCC1 (Ser11) Monoclonal antibody (60089-1-Ig)

    IHC staining of human testis using 60089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 60089-1-Ig(phospho-RCC1(Ser12) antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using Phospho-RCC1 (Ser11) Monoclonal antibody (60089-1-Ig)

    IHC staining of human testis using 60089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 60089-1-Ig(phospho-RCC1(Ser12) antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Phospho-RCC1 (Ser11) Monoclonal antibody (60089-1-Ig)

    IHC staining of human ovary tumor using 60089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 60089-1-Ig(phospho-RCC1(Ser12) antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Phospho-RCC1 (Ser11) Monoclonal antibody (60089-1-Ig)

    IHC staining of human ovary tumor using 60089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 60089-1-Ig(phospho-RCC1(Ser12) antibody) at dilution of 1:50 (under 40x lens).

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60089-1-Ig targets phospho-RCC1(Ser11) in ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen peptide
Full Name regulator of chromosome condensation 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberNM_001048194
Gene Symbol RCC1
Gene ID (NCBI) 1104
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a mouse monoclonal antibody raised against human phospho-CHC1(Ser11) antigen.

Protocols

Product Specific Protocols
IHC protocol for phospho-RCC1(Ser11) antibody 60089-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols