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Presented by Proteintech product and technical support manager Dr Karolina Szczesna, this webinar gives you technical tips and advice on how to optimize your immunofluorescence experiments to achieve the best results.
Immunofluorescence (IF) staining is a widely used technique in biological research and clinical diagnostics. IF utilizes fluorescent-labeled antibodies in order to detect specific target antigens. Followed by imaging, it is a very direct technique as you can actually see something. Although it is a well-established tool, multiple factors have to be considered and various optimization steps have to be taken to ensure successful staining.
Brief overview of the IF technique
Things to consider before starting your IF experiment
IF Troubleshooting (No/weak signal/unspecific staining/background staining)
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