For Research Use Only
GGGGS Recombinant Monoclonal Antibody
Catalog Number:CT-1002
www.ptglab.com
Basic Information
Catalog Number:
CT-1002
AntigenName:
GGGGS
Immunogen:
KLH conjugated GGGGSGGGGSGGGGS
Isotype:
Rabbit IgG
Reactivity:
human
Conjugate:
Unconjugated
Clonality:
Recombinant
CloneNo:
Application:
Cell Activation
Formulation:
1XPBS
Format:
Liquid
Product Specifications
| Test | Method | Specification |
|---|---|---|
| Endotoxin | Tested in accordance with USP <85> | <0.1 EU/μg |
| Identity | SDS-PAGE | 25kDa and 50kDa bands in reducing conditions |
| Mycoplasma | tested in accordance with USP <63> | Not Detected |
| Purity | SDS-PAGE followed by denitometric analysis | >95% |
| Specificity | T cell activation | >5-fold over unactivated |
Background
As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S, G4S*2, G4S*3, G4S*4, and G4S*5.
Storage
Storage:
Store at -20°C to -80°C
Storage Buffer:
1XPBS
Blue ice
For technical support and original validation data for this product please contact:
T: 1 (888) 4PTGLAB
(1-888-478-4522)
(toll free in USA),
or 1(312) 455-8498
(outside USA)
E: proteintech@ptglab.com
W: ptglab.com
W: ptglab.com
This product is exclusively available under
Proteintech Group brand and is not available
to purchase from any other manufacturer.
Selected Validation Data
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0.5x10^6 GGGGS-transfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002) (red) or Rabbit IgG Isotype Control (98136-1-RR) (blue). 0.5x10^6 untransfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002-GMP) (black dashed). All cells were then stained with CL647-conjugated Donkey Anti-Rabbit Secondary (SA00014-7) at 1:500. Propidium Iodide was included in the final resuspension buffer at 1:1000. Cells were gated on Live (PI-), then the cell population in FSC vs. SSC, then single cells, then for positive signal in the APC-A channel.
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0.5x10^6 GGGGS-transfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002) (red) or Rabbit IgG Isotype Control (98136-1-RR) (blue). 0.5x10^6 untransfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002-GMP) (black dashed). All cells were then stained with CL647-conjugated Donkey Anti-Rabbit Secondary (SA00014-7) at 1:500. Propidium Iodide was included in the final resuspension buffer at 1:1000. Cells were gated on Live (PI-), then the cell population in FSC vs. SSC, then single cells, then for positive signal in the APC-A channel.
-
0.5x10^6 GGGGS-transfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002) (red) or Rabbit IgG Isotype Control (98136-1-RR) (blue). 0.5x10^6 untransfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002-GMP) (black dashed). All cells were then stained with CL647-conjugated Donkey Anti-Rabbit Secondary (SA00014-7) at 1:500. Propidium Iodide was included in the final resuspension buffer at 1:1000. Cells were gated on Live (PI-), then the cell population in FSC vs. SSC, then single cells, then for positive signal in the APC-A channel.