Tested Applications
| Positive WB detected in | NIH/3T3 cells, THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31001-1-AP targets LRMDA in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LRMDA fusion protein Ag33898 Predict reactive species |
| Full Name | chromosome 10 open reading frame 11 |
| Calculated Molecular Weight | 23 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | NM_032024 |
| Gene Symbol | LRMDA/C10orf11 |
| Gene ID (NCBI) | 83938 |
| RRID | AB_3669812 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H2I8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The LRMDA gene, also known as C10orf11, encodes a leucine-rich repeating protein that is thought to play a role in melanocyte differentiation. Variants in this gene have been associated with autosomal recessive oculocutaneous albinism 7 (OCA7), but have never been implicated in metabolic disorders. Interestingly, however, the variant associated with age at diabetes onset is also associated with differences in LRMDA expression in the brain, including the hypothalamus, where peptides controlling pigmentation, such as melanocortins and their receptors, are involved in the regulation of body weight and metabolism. (PMID: 36104811)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LRMDA antibody 31001-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
