ACY1 Polyclonal antibody

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  WB analysis using 11316-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11316-1-AP (Aminoacylase 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11316-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11316-1-AP (Aminoacylase 1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 11316-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11316-1-AP (Aminoacylase 1 antibody at dilution of 1:50 (under 10x lens).

ACY1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11316-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ACY 1, ACY-1, aminoacylase 1, Aminoacylase-1, EC:3.5.1.14

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Tested Applications

• Positive WB detected in: K-562 cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11316-1-AP targets ACY1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACY1 fusion protein Ag1813
• Full Name: aminoacylase 1
• Calculated Molecular Weight: 40.8 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC014112
• Gene Symbol: ACY1
• Gene ID (NCBI): 95
• RRID: AB_2877757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q03154
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Heliyon; FYN-mediated phosphorylation of BCKDK at Y151 promotes GBM proliferation by increasing the oncogenic metabolite N-acetyl-L-alanine; Authors - Ling Zou