PLAP Polyclonal antibody

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• KD/KO Validated

PLAP Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 18507-1-AP

Synonyms

ALP, ALPP, PALP, Placental alkaline phosphatase, PLAP, PLAP 1

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Validation Data Gallery

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  WB analysis of human placenta using 18507-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18507-1-AP (PLAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18507-1-AP

  WB result of PLAP antibody (18507-1-AP, 1:500) with si-control and si-PLAP transfected HepG2 cells.

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  WB analysis of mouse liver using 18507-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18507-1-AP (PLAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 18507-1-AP

  IP result of anti-PLAP (IP:18507-1-AP, 4ug; Detection:18507-1-AP 1:700) with HepG2 cells lysate 1800ug.

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  IHC staining of human placenta using 18507-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18507-1-AP (PLAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18507-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18507-1-AP (PLAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 18507-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18507-1-AP (PLAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 18507-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PLAP (18507-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse liver tissue, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 18507-1-AP is PLAP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLAP fusion protein Ag13361
• Full Name: alkaline phosphatase, placental (Regan isozyme)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC009647
• Gene Symbol: ALPP
• Gene ID (NCBI): 250
• RRID: AB_10644334
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.

Publications

Species	Application	Title
mouse	WB	Aging (Albany NY); Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway.; Authors - Jie Xu
human	IHC	ACS Biomater Sci Eng; 6-Bromoindirubin-3'-oxime Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells and Facilitates Bone Regeneration in a Mouse Periodontitis Model.; Authors - Song Shen
mouse	WB	Mol Cell Endocrinol; Long noncoding RNA ZFAS1 suppresses osteogenic differentiation of bone marrow-derived mesenchymal stem cells by upregulating miR-499-EPHA5 axis.; Authors - Jinhui Wu
	WB	J Dent; PMID: 32119967; Authors - Yunxia Zhang
human	WB	Amino Acids; New aspects of antiproliferative activity of 4-hydroxybenzyl isothiocyanate, a natural H2S-donor.; Authors - Halina Jurkowska
mouse	WB	Biomaterials; NRF2 is a critical regulator and therapeutic target of metal implant particle-incurred bone damage; Authors - Jian Dong

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C