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APOL1-Specific Monoclonal antibody

APOL1-Specific Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1G12D11

Cat no : 66124-1-Ig

Synonyms

APO L, APOL, APOL I, APOL1, APOL1-Specific, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:5000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66124-1-Ig targets APOL1-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen APOL1-Specific fusion protein Ag2016
Full Name apolipoprotein L, 1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC017331
Gene Symbol APOL1
Gene ID (NCBI) 8542
RRIDAB_2881523
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.

Protocols

Product Specific Protocols
WB protocol for APOL1-Specific antibody 66124-1-IgDownload protocol
IHC protocol for APOL1-Specific antibody 66124-1-IgDownload protocol
IF protocol for APOL1-Specific antibody 66124-1-IgDownload protocol
IP protocol for APOL1-Specific antibody 66124-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IF

Immunity

APOL1 risk variants in individuals of African genetic ancestry drive endothelial cell defects that exacerbate sepsis.

Authors - Junnan Wu
WB

J Clin Invest

The key role of NLRP3 and STING in APOL1-associated podocytopathy.

Authors - Junnan Wu
humanWB

J Am Soc Nephrol

Kidney Disease-Associated APOL1 Variants Have Dose-Dependent, Dominant Toxic Gain-of-Function.

Authors - Somenath Datta
WB

Mol Ther

Antisense oligonucleotides ameliorate kidney dysfunction in podocyte-specific APOL1 risk variant mice.

Authors - Ya-Wen Yang
humanWB,IF

Elife

Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity.

Authors - Joseph A Giovinazzo
humanWB,IHC

Neurobiol Dis

Apolipoprotein L1 is increased in frontotemporal lobar degeneration post-mortem brain but not in ante-mortem cerebrospinal fluid.

Authors - Yanaika S Hok-A-Hin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jonathan (Verified Customer) (11-11-2019)

Works well for WB.

  • Applications: Western Blot,