CARHSP1 Recombinant antibody

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  WB analysis using 83300-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83300-3-RR (CARHSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 83300-3-RR

  WB result of CARHSP1 antibody (83300-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARHSP1 transfected HEK-293 cells.

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  FC experiment of HepG2 using 83300-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-3-RR, Clone:230489A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CARHSP1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83300-3-RR

Clone No.230489A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HepG2 cells, HEK-293T cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83300-3-RR targets CARHSP1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CARHSP1 fusion protein Ag2265
• Full Name: calcium regulated heat stable protein 1, 24kDa
• Calculated Molecular Weight: 147 aa, 16 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC003366
• Gene Symbol: CARHSP1
• Gene ID (NCBI): 23589
• RRID: AB_3670970
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y2V2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARHSP1(calcium-regulated heat-stable protein 1, a 24 kDa protein also known as CRHSP-24) is a ubiquitously expressed phosphoprotein that is comprised of 147 amino acids with nearly 14% proline (PMID: 21990353,30257675). CARHSP1 was discovered as a biomarker for diabetic retinopathy and as a regulator of TNF-α mRNA stability in macrophages (PMID: 21078874). CARHSP1 may be involved in oxidative stress via traffic between stress granules and processing bodies (PMID: 21177848).