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CoraLite® Plus 647-conjugated CDC27; APC3 Monoclonal antibody

CDC27; APC3 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2F10G2

Cat no : CL647-67239

Synonyms

ANAPC3, APC3, CDC27, CDC27 homolog, CDC27Hs, D0S1430E, D17S978E, H NUC, HNUC

Validation Data Gallery

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  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 647-conjugated CDC27; APC3 Monoclon (CL647-67239)

    FC experiment of K-562 using CL647-67239

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human CDC27; APC3 (CL647-67239, Clone:2F10G2) (red), or 0.4 ug CoraLite® Plus 647 Mouse IgG2b Isotype Control (MPC-11) (CL647-65128, Clone: MPC-11) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67239 targets CDC27; APC3 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CDC27; APC3 fusion protein Ag25588
Full Name cell division cycle 27 homolog (S. cerevisiae)
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC011656
Gene Symbol CDC27
Gene ID (NCBI) 996
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.